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Image Search Results
Journal: Cells
Article Title: Modulation of Suppressive Activity and Proliferation of Human Regulatory T Cells by Splice-Switching Oligonucleotides Targeting FoxP3 Pre-mRNA.
doi: 10.3390/cells13010077
Figure Lengend Snippet: Figure 10. The ability of Tregs to produce apoptosis-inducing molecules (A) granzyme A, (B) granzyme B, and (C) perforin. Transfected Tregs were incubated with stimulators and pro- tein transport inhibitors or only with protein transport inhibitors as a control. Cells were labeled with CD4, CD25, and CD127 antibodies, fixed, permeabilized, and incubated with antibodies against granzyme A, B, or perforin. Levels of marker-positive cells were measured by flow cytometry. n = 4. Black horizontal lines indicate the mean ± SD. * p ≤0.05; ** p ≤0.01; *** p ≤0.005 vs. cells transfected with #Con1 & #Con2 oligonucleotides by the Mann–Whitney U test. Representative flow cytometry diagrams for granzymes A and B and perforin are shown in Figure S15 in the Supplementary File.
Article Snippet: After stimulation, the cells were fixed and permeabilized as described above and labeled with
Techniques: Transfection, Incubation, Control, Labeling, Marker, Flow Cytometry, MANN-WHITNEY